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pethis6tevlic1b addgene  (Addgene inc)


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    Structured Review

    Addgene inc pethis6tevlic1b addgene
    Pethis6tevlic1b Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+plasmid+29653+1b/pET+His6+TEV+LIC+cloning+vector+(1B)+(Plasmid+%2329653)/pm41161312-272-242-243
    Average 94 stars, based on 119 article reviews
    pethis6tevlic1b addgene - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Temporal and fluoride control of secondary metabolism regulates cellular organofluorine biosynthesis
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). .. Following plasmid construction, all cloned inserts were sequenced at Sequetech (Mountainview, CA) or Quintara Biosciences (Berkeley, CA).

    Article Title: Expanding the scope of organofluorine biochemistry through the study of natural and engineered systems
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). ..

    Amplification:

    Article Title: Temporal and fluoride control of secondary metabolism regulates cellular organofluorine biosynthesis
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). .. Following plasmid construction, all cloned inserts were sequenced at Sequetech (Mountainview, CA) or Quintara Biosciences (Berkeley, CA).

    Article Title: Expanding the scope of organofluorine biochemistry through the study of natural and engineered systems
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). ..

    Plasmid Preparation:

    Article Title: Temporal and fluoride control of secondary metabolism regulates cellular organofluorine biosynthesis
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). .. Following plasmid construction, all cloned inserts were sequenced at Sequetech (Mountainview, CA) or Quintara Biosciences (Berkeley, CA).

    Article Title: Expanding the scope of organofluorine biochemistry through the study of natural and engineered systems
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). ..

    Ligation:

    Article Title: Temporal and fluoride control of secondary metabolism regulates cellular organofluorine biosynthesis
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). .. Following plasmid construction, all cloned inserts were sequenced at Sequetech (Mountainview, CA) or Quintara Biosciences (Berkeley, CA).

    Article Title: Expanding the scope of organofluorine biochemistry through the study of natural and engineered systems
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). ..

    Cloning:

    Article Title: Temporal and fluoride control of secondary metabolism regulates cellular organofluorine biosynthesis
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). .. Following plasmid construction, all cloned inserts were sequenced at Sequetech (Mountainview, CA) or Quintara Biosciences (Berkeley, CA).

    Article Title: Expanding the scope of organofluorine biochemistry through the study of natural and engineered systems
    Article Snippet: .. 1b-His6-Cit2.Scat was constructed by amplification out of the appropriate genomic DNA and inserted into the SspI site of Addgene plasmid 29653 (1b) by Ligation Independent Cloning (LIC) by the QB3 MacroLab (Berkeley, CA). ..



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    Image Search Results


    Journal: Cell

    Article Title: Structural basis for the assembly of the type V CRISPR-associated transposon complex

    doi: 10.1016/j.cell.2022.11.009

    Figure Lengend Snippet:

    Article Snippet: The ShCas12k gene was inserted into the 1B (Addgene #29653) and 1R (Addgene #29664) plasmids using ligation-independent cloning (LIC), resulting in constructs carrying an N-terminal hexahistidine tag followed by a tobacco etch virus (TEV) protease cleavage site and an N-terminal hexahistidine-StrepII tag followed by a TEV cleavage site, respectively.

    Techniques: Recombinant, Mass Spectrometry, Electron Microscopy, Plasmid Preparation, Virus, Cloning, Software